Tris-Glycine Transfer Buffer (10X) #12539
Product Information
Product Usage Information
To make 1L of 1X transfer buffer: Mix 100 ml of 10X transfer buffer, 200 ml of methanol, and 700 ml of ddH2O and store at 4ºC for up to one week. This product supplies enough 10X material to make 10 liters of 1X solution. Generally, 20% methanol is recommended, however it may be beneficial to decrease methanol concentration to 5-10% for increased transfer efficiency of large, low abundancy proteins. CST recommends electrotransferring to 0.2 μm pore size nitrocellulose membranes at 70 volts for 2 hours.
Storage
Product Description
1X Formulation: 25 mM Tris, 192 mM Glycine, 20% (v/v) methanol, pH ~8.3.
Note: Methanol is not supplied but is required.
Background
Limited Uses
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