SignalStain® Vivid Purple Peroxidase Substrate Kit #96632
Product Information
Product Usage Information
1. To prepare the Vivid Purple working solution, to 5 ml SignalStain® Vivid Purple Diluent add 3 drops SignalStain® Vivid Purple Reagent 1, 3 drops SignalStain® Vivid Purple Reagent 2, 3 drops SignalStain® Vivid Purple Reagent 3, and 3 drops SignalStain® Vivid Purple Reagent 4. Mix well before use.
2. After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature for 2-15 minutes. Optimal development time should be determined by the investigator.
3. Wash slides for 5 minutes in water.
4. Counterstain, if desired.
5. Dehydrate, clear and permanently mount in a non-aqueous mounting medium. Do not use recycled alcohols with this product.
Prolonged incubation in alcohol may decrease sensitivity.
Unused working solution is stable up to 14 days if stored at 2-8ºC. Working solution may change color during this time with no impact on quality or intensity of the staining. If a precipitate forms, filter through a 0.45 μm filter.
Appropriate personal protective equipment should be worn when handling Vivid Purple. Dispose of in accordance with local regulations.
Storage
Product Description
Limited Uses
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