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Product last modified at: 2024-11-15T15:00:10.465Z
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PDP - Template Name: Assay Kit One Column
PDP - Template ID: *******68700a6
  1. Products /
  2. Cellular Assay Kits /
  3. CAR (G4S Linker) Cell Enrichment Kit

CAR (G4S Linker) Cell Enrichment Kit #48669

    Product Information

    Protocol

    Product Description

    The CAR (G4S Linker) Cell Enrichment Kit is designed to positively select cells that are engineered to express a G4S linker-containing single-chain variable fragment (scFv)-based chimeric antigen receptor (CAR) on the cell surface. Using an indirect magnetic bead-based immunoaffinity enrichment protocol, this kit yields highly pure and viable CAR positive cells that are bead-free. Within a heterogeneous population of cells, desired CAR expressing cells are labeled with a biotinylated rabbit monoclonal antibody targeting the G4S linker. Releasable Streptavidin Beads are then incubated with the cell mixture. Bead-bound cells are isolated without columns using a magnet (not included), and then beads are removed from cells using DNase I. Following release from beads, the positively selected fraction of CAR positive cells containing surface-bound biotinylated antibody-streptavidin complexes are available for expansion or use in downstream applications such as flow cytometry and gene expression analyses.

    Bead Specifications:
    Releasable Streptavidin Beads are paramagnetic polystyrene beads (4.5 μm diameter) coupled to streptavidin via a DNA linker susceptible to cleavage by DNase I. The beads provided in this kit are not recommended for the isolation of phagocytic cells as the beads are likely to be engulfed by these cells.

    Specificity / Sensitivity

    Biotinylated G4S Linker (E7O2V) Rabbit mAb recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.

    Species Reactivity:

    All Species Expected

    Background

    Magnetic bead-based immunoaffinity cell enrichment is a gentle purification method that can be leveraged, in part, to facilitate a robust interrogation of the biology of immune cells that are engineered to express CARs. For example, isolation of rare subsets of CAR positive cells from a complex, heterogeneous population of cells presents the opportunity for extensive downstream analyses using single-cell omics assays. In the context of CAR cell engineering, magnetic bead-based immunoaffinity cell enrichment can also be useful in scenarios when delivery of the CAR transgene is inefficient, resulting in a small population of cells expressing the CAR transgene.

      Limited Uses

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      Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

      For Research Use Only. Not For Use In Diagnostic Procedures.
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