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  1. Products /
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  3. Cellular Reactive Oxygen Species (ROS) Detection Assay Kit

Cellular Reactive Oxygen Species (ROS) Detection Assay Kit #13323

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    Product Information

    Product Description

    The Cellular Reactive Oxygen Species (ROS) Detection Assay Kit is a fluorescent assay that detects overall ROS levels in living cells. The kit contains the non-fluorescent fluorescein derivative DCFH-DA, a common ROS inducer tert-butyl hydroperoxide (TBHP), Loading Buffer, and 20X PBS wash buffer. DCFH-DA diffuses into cells during pre-incubation and deacetylated by cellular esterases to DCFH, which can be rapidly oxidized to the highly fluorescent DCF by cellular ROS. The DCF fluorescence is used to quantify overall levels of cellular ROS. The kit includes sufficient reagents for 500 assays in a 96-well plate, or 50 flow cytometry assays.

    Specificity / Sensitivity

    The Cellular Reactive Oxygen Species (ROS) Detection Assay Kit is expected to detect reactive oxygen species in living cells across all species. A cell number titration is recommended when using a plate-reader with 96-well plate.

    Species Reactivity:

    All Species Expected

    Background

    Reactive oxygen species (ROS) include a variety of highly reactive oxidant molecules and free radicals that are derived from molecular oxygen. ROS are generated as a result of both normal cellular metabolism and environmental factors including air pollutants or cigarette smoking. The major ROS of physiological significance are superoxide anions (O2-.), hydroxyl radicals (. OH), and hydrogen peroxide (H2O2) (1-3). Low to moderate ROS levels play important roles in normal physiological processes including vascular tone regulation, oxygen sensing, immunological reaction, regulation of translation, and signal transduction. High ROS concentrations can lead to adverse modifications of cellular components, such as carbohydrates, lipids, proteins, and DNA. Many of these modifications have been linked to diseases including atherosclerosis, diabetes, cancer, and neurological disorders. As a result, regulation of reducing and oxidizing (redox) state is not only critical for cell health, but is also an attractive therapeutic target for many diseases (1-3).

    2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is a non-fluorescent fluorescein derivative used as a non-ionic, non-polar, cell membrane permeable dye. After crossing the cell membrane, DCFH-DA is hydrolyzed by intracellular esterases to a non-fluorescent dichlorodihydrofluorescein (DCFH). DCFH is relatively more polar and cell membrane-impermeable and thus accumulates in cells. In the presence of cellular ROS, DCFH is oxidized to the highly fluorescent dichlorofluorescein (DCF). The fluorescent intensity of DCF is proportional to the overall cellular ROS level (4,5).

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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