Product Information

Product Description

The kit provides a means of performing kinase activity assays with recombinant murine Jak2 kinase. It includes active Jak2 kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-tyrosine antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula

FLT3 (Tyr589): 1,945 Daltons, GST-Jak2 Kinase: 71 kDa Daltons.

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

Database Links

UniProt ID: O60674

Entrez-Gene Id: 3717

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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HTScan® Jak2 Kinase Assay Kit #7752