Mitochondrial Membrane Potential Assay Kit (II) #13296
- F
To Purchase # 13296
| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 13296S | 1 Kit 500 assays (96 well format) | $356 |
- Product Includes
- Related Products
| Product Includes | Solution Color | Quantity (with Count) |
|---|---|---|
| TMRE #13472 | Purple | 1 x 29 µg |
| CCCP #13550 | Yellow | 1 x 100 µl |
| Phosphate Buffered Saline (PBS-20X) #9808 | 1 x 25 ml |
Product Information
Protocol
Product Description
Specificity / Sensitivity
Species Reactivity:
Background
TMRE (tetramethylrhodamine, ethyl ester) is a cell membrane permeable cationic dye. In normal cells, TMRE accumulates in the mitochondria in response to their high membrane potential and negative charge. When excited at 550 nm, TMRE emits an orange-red fluorescence with a maximum at 575 nm (orange-red). Cells that have lost membrane potential or mitochondria activity cannot accumulate TMRE. Therefore, the fluorescence intensity of the orange-red emission can be used to measure mitochondria membrane potential and is an indicator for cell health (4).
Limited Uses
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