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Serine/Threonine Kinase Substrate Screening Kit

Serine/Threonine Kinase Substrate Screening Kit #7400

Inquiry Info. # 7400

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Product Information

Product Description

The Serine/Threonine Kinase Substrate Screening Kit provides 87 biotinylated nonphospho-peptides as kinase substrates. Also included are biotinylated phospho and nonphospho-peptides of identical sequence as positive and negative controls respectively. Potential substrate phosphorylation is detected by the corresponding phospho-specific antibodies.
The paired phospho-specific antibody and peptide substrate can be used for high throughput peptide based kinase screens (e.g. FP, TRF, AlphaScreen).

Specificity / Sensitivity

10 to 1000+ fold signal-to-noise ratio of phospho- versus nonphospho-peptides measured by DELFIA^® assay.

Background

In order to expedite the isolation of substrates for novel kinases, Cell Signaling Technology has developed a kinase/substrate development kit. Numerous serine/threonine kinases have become pharmaceutically important, including GSK, Akt and p38. With the cloning of hundreds of uncharacterized serine/threonine kinases, drug discovery researchers are seeking to quickly find substrates for high throughput screening programs.
With a total of 87 different peptide/phospho-specific antibody pairs on the array, a very diverse group of substrates is represented. Signal-to-noise ratios have been optimized and range from 10 to over 1000. The phospho-specific antibody and peptide substrate pair can be used for subsequent high throughput peptide based kinase screens (e.g., FP, TRF, AlphaScreen).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

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