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CUT&Tag PCR Master Mix

CUT&Tag PCR Master Mix #63228

Name: CUT&Tag PCR Master Mix
Price: 64.00 USD
Availability: InStock

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Product Information

Storage

Store at -20ºC. This product is stable for 18 months if stored properly.

Protocol

Product Description

The CUT&Tag PCR Master Mix is an optimized 2X reaction mix for PCR amplification and DNA library preparation for CUT&Tag DNA samples. The non hot-start DNA polymerase in this product ensures the success of gap filling extension for tagmentated DNA. This master mix formulation is supplied at 2X concentration and contains all PCR components required for amplification of DNA, except primers and a DNA template. This product is provided in 840 μL volumes sufficient for preparation of 24 PCR reactions, and is compatible with CUT&Tag DNA sample generated by CUT&Tag Assay Kit #77552 or CUT&Tag pAG-Tn5 (Loaded) #79561 and the index primers provided in the CUT&Tag Dual Index Primers and PCR Master Mix for Illumina Systems #47415. This product is not compatible with library preparation for DNA samples from SimpleChIP^® Chromatin IP Kits (#9003, #9005, #56383) or the CUT&RUN Assay Kit #86652.

Specificity / Sensitivity

Species Reactivity:

All Species Expected

Background

Similar to Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag) is a powerful technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1-3). CUT&Tag has many of the same advantages as the CUT&RUN assay in that it provides a rapid, robust, and true low cell number protocol for detection of protein-DNA interactions in the cell. In addition, the CUT&Tag assay adds an in situ adaptor DNA ligation step carried out by the pAG-Tn5 enzyme, in which an adaptor DNA is ligated directly to antibody-targeted chromatin DNA fragments in the cell. As a result, subsequent DNA library preparation is much faster and easier than library preparation following the CUT&RUN assay, free from DNA end repair, A-tailing, and adaptor ligation in vitro. CUT&Tag works very well for analyzing histone modifications, in addition to mapping some transcription factor and cofactor binding.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

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