Human β-NGF Recombinant Protein #84087
Product Information
Storage
Human β-NGF Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 27.3 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hβ-NGF. |
| Activity | The bioactivity of recombinant hβ-NGF was determined in a TF-1 cell proliferation assay. The ED50 of each lot is less than or equal to 5 ng/mL. |
Source / Purification
Background
- Bibel, M. and Barde, Y.A. (2000) Genes Dev 14, 2919-37.
- Huang, E.J. and Reichardt, L.F. (2003) Annu Rev Biochem 72, 609-42.
- Frossard, N. et al. (2004) Eur J Pharmacol 500, 453-65.
- Jiang, Y. et al. (2007) J Immunol 179, 6297-304.
- Matrone, C. et al. (2009) Proc Natl Acad Sci U S A 106, 11358-63.
- Matrone, C. et al. (2008) Proc Natl Acad Sci U S A 105, 13139-44.
Limited Uses
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