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XML generation date: 2024-04-05 20:41:27.640
Product last modified at: 2024-06-11T12:30:10.077Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human BAFF/TNFSF13B (hBAFF) Recombinant Protein #89628

    Product Information

    Storage

    Recombinant human BAFF is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 18.5
    Purity A greater than 90% purity was determined by SDS-PAGE.
    Endotoxin Less than or equal to 1 EU / 1 μg hBAFF.

    Source / Purification

    Recombinant human BAFF was expressed in E. coli and is supplied in a lyophilized form.

    Background

    BAFF, a member of the TNF superfamily of proteins, is a homotrimeric transmembrane protein, which is cleaved to produce a soluble cytokine (1). BAFF may also further oligomerize into 60-mer structures (1). BAFF is expressed by neutrophils, macrophages, dendritic cells, activated T cells, and epithelial cells (1,2). BAFF plays a key role in B cell development, survival, and activation (1,3,4). BAFF binds to three distinct receptors, BAFF-R, TACI, and BCMA (1). These receptors are differentially expressed during B cell development and among B cell subsets (1,2,4). While BAFF-R and BCMA bind to the homotrimeric form of BAFF, TACI only binds to membrane bound or higher order BAFF structures (1). The BAFF/ BAFF-R interaction activates both canonical and non-canonical NF-κB pathways, PI3K/Akt, and mTOR (2,4). Activation of the noncanonical NF-κB pathway via BAFF-R is negatively regulated by TRAF3 (5). Elevated levels of BAFF may exacerbate many autoimmune disorders, making it an attractive therapeutic target (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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