Human CSF-1/M-CSF Recombinant Protein #8929
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Inquiry Info. # 8929
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Product Information
Product Usage Information
Reconstitution:
With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final hCSF-1/M-CSF concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.
Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of hCSF-1/M-CSF to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hCSF-1/M-CSF should be greater than 50 μg/ml.
Formulation
Storage
Product Description
| MW (kDa) | 16-26 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCSF-1/M-CSF. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg hCSF-1/M-CSF. |
| Activity | The bioactivity of recombinant hCSF-1M/CSF was determined in a M-NFS-60 cell proliferation assay. The ED50 of each lot is between 0.5-15.0 ng/ml. |
| Molecular Formula | Recombinant hCSF-1/M-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,403. DTT-reduced protein migrates as a 16-26 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 34-40 kDa protein. Heterogeneity in SDS PAGE is due to glycosylation. The expected amino-terminal EEVSE of recombinant hCSF-1/M-CSF was verified by amino acid sequencing. |
Source / Purification
Background
Product Citations: 1
Limited Uses
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