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XML generation date: 2024-11-21 19:05:07.522
Product last modified at: 2024-11-21T17:45:08.977Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159
  1. Products /
  2. Cytokines & Growth Factors /
  3. Human EGF Recombinant Protein

Human EGF Recombinant Protein #72528

    Product Information

    Storage

    Human EGF Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 6.2
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hEGF.
    Activity The bioactivity of recombinant hEGF was determined in a BALB/c 3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 100 pg/mL.

    Source / Purification

    Recombinant human EGF was expressed in E. coli and is supplied in a lyophilized form.

    Background

    Epidermal growth factor (EGF) is a small polypeptide hormone that has mitogenic properties in vivo and in vitro and affects the growth and/or differentiation of many cell types. EGF elicits biological responses by binding to its cell surface receptor, which is a transmembrane glycoprotein containing a cytoplasmic protein tyrosine kinase (PTK) (1,2). The binding of EGF to EGF receptor induces dimerization of the receptor, autophosphorylation, and activation of downstream signaling components (3). The integrated biological responses to EGF signaling are pleiotropic, including mitogenesis or apoptosis, enhanced cell motility, protein secretion, and differentiation or dedifferentiation. In addition to being implicated in organ morphogenesis, maintenance, and repair, research studies have correlated upregulated EGF receptor signaling with progression to invasion and metastasis in a wide variety of tumors (4-6). Thus, investigators have identified EGF receptor and its downstream signaling molecules as targets for therapeutic interventions in wound repair and cancer (4-6). EGF is derived from a 160 kDa precursor protein with an N-terminal extracellular domain, an EGF growth factor sequence, a transmembrane domain, and an extracellular tail (7). Once synthesized, the precursor can be stored or processed into EGF growth factor depending on different tissue/organ conditions.

    Database Links

    UniProt ID: P01133


    Entrez-Gene Id: 1950


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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