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Human FGF-4 Recombinant Protein #53943

Human FGF-4 Recombinant Protein: Image 1
Serial dilutions of Human FGF-4 Recombinant Protein were added to NR6R-3T3 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.

To Purchase # 53943

Cat. # Size Qty. Price Ships
53943S 25 µg
$276

Product Information

Storage

Human FGF-4 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

Product Description

MW (kDa) 19.4
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hFGF-4.
Activity The bioactivity of recombinant hFGF-4 was determined in an NR6R-3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 5 ng/mL.

Source / Purification

Recombinant human FGF-4 was expressed in E. coli and is supplied in a lyophilized form.

Background

FGF-4 is a member of the large and diverse fibroblast growth factor (FGF) family of peptide growth factors. FGF-4 functions as a signaling molecule that is involved in many important processes (1,2). FGF-4 binds to FGFR1 and is required for cardiac gene expression, particularly during second heart field (SHF) proliferation and chamber formation in cooperation with the Sonic hedgehog gene (Shh) during heart development (3,4). FGF-4 knockout mice showed developmental defects in embryos thus suggesting that FGF-4 facilitates survival and growth of the inner cell mass during the postimplantation phase of development (1). Since FGFs are involved in gastrointestinal development, upregulation of FGF-4 in pluripotent stem cells has been used to direct their differentiation for the generation of intestinal organoids in vitro (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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