Human FGF-basic/FGF2 (147 aa) Recombinant Protein #42430
Product Information
Storage
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 16.5 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hFGF-basic. |
| Activity | The bioactivity of recombinant human FGF-basic was determined in a NR6R-3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 5 ng/mL. |
Source / Purification
Recombinant human FGF-basic was expressed in E. coli and is supplied in a lyophilized form.
Background
FGF-basic (FGF2) is produced by epithelial, tumor, and other cell types (1). FGF-basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF-basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF-basic has a high affinity for heparan sulfate (1,2) and FGF-heparan sulfate binding is a step in the activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF-basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF-basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/Akt pathways (1).
- Dvorak, P. and Hampl, A. (2005) Folia Histochem Cytobiol 43, 203-8.
- Ornitz, D.M. and Itoh, N. (2001) Genome Biol 2, REVIEWS3005.
- Shi, Y. et al. (2008) Crit Rev Oncol Hematol 65, 43-53.
- Fontijn, D. et al. (2006) Br J Cancer 94, 1627-36.
- Marek, L. et al. (2009) Mol Pharmacol 75, 196-207.
- Acevedo, V.D. et al. (2009) Cell Cycle 8, 580-8.
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