Human GDF5 Recombinant Protein #72306
Product Information
Storage
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 27.4 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hGDF5. |
| Activity | The bioactivity of recombinant hGDF5 was determined by measuring the alkaline phosphatase activity in ATDC-5 cells. The ED50 of each lot is less than or equal to 1.2 μg/mL. |
Source / Purification
Background
Growth differentiation factor 5 (GDF5), also known as BMP-14, belongs to the BMP family of transforming growth factor beta (TGF-β) superfamily (5). GDF plays a critical role in skeletal, limb, and joint development (6,7). Decreased or altered expression of GDF5 leads to the pathogenesis of osteoarthritis, as well as Parkinson's disease (7-9).
- Wang, E.A. et al. (1988) Proc Natl Acad Sci U S A 85, 9484-8.
- Wozney, J.M. et al. (1988) Science 242, 1528-34.
- Kawabata, M. et al. (1998) Cytokine Growth Factor Rev 9, 49-61.
- Nohe, A. et al. (2004) Cell Signal 16, 291-9.
- Chang, S.C. et al. (1994) J Biol Chem 269, 28227-34.
- Buxton, P. et al. (2001) J Bone Joint Surg Am 83-A Suppl 1, S23-30.
- Degenkolbe, E. et al. (2013) PLoS Genet 9, e1003846.
- Zhang, Y. et al. (2014) Exp Mol Med 46, e79.
- Sullivan, A.M. and O'Keeffe, G.W. (2005) J Anat 207, 219-26.
Limited Uses
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