Human Granulocyte Colony Stimulating Factor (hG-CSF) #8930
Product Information
Product Usage Information
Reconstitution:
With carrier: Add sterile 40 mM phosphate pH 4.0 to a final hG-CSF concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.
Carrier free: Add sterile 40 mM phosphate pH 4.0, or 40 mM phosphate pH 4.0 containing protein to minimize absorption of hG-CSF to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hG-CSF should be greater than 50 μg/ml.
Formulation
Storage
Product Description
| MW (kDa) | 18 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hG-CSF. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1μg hG-CSF. |
| Activity | The bioactivity of recombinant hG-CSF was determined in a M-NFS-60 cell proliferation assay. The ED50 of each lot is between 20-150 pg/ml. |
| Molecular Formula | Recombinant hG-CSF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,986. DTT-reduced and non-reduced protein migrate as 18 kDa polypeptides. The expected amino-terminal TPLGP of recombinant hG-CSF was verified by amino acid sequencing. |
Source / Purification
Background
- Furze, R.C. and Rankin, S.M. (2008) Immunology 125, 281-8.
- Demetri, G.D. and Griffin, J.D. (1991) Blood 78, 2791-808.
- Srinivasa, S.P. and Doshi, P.D. (2002) Leukemia 16, 244-53.
- van Raam, B.J. et al. (2008) Blood 112, 2046-54.
- Schneider, A. et al. (2005) J Clin Invest 115, 2083-98.
- Nicholson, S.E. et al. (1994) Proc Natl Acad Sci U S A 91, 2985-8.
- Lieschke, G.J. et al. (1994) Blood 84, 1737-46.
- Dong, F. et al. (1994) Proc Natl Acad Sci U S A 91, 4480-4.
Product Citations: 1
Limited Uses
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