Human IFN-α 2a Recombinant Protein #93613
Product Information
Storage
Human IFN-α 2a Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 19.4 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hIFN-α 2a. |
| Activity | The bioactivity of recombinant hIFN-α 2a was determined in a viral CPE assay using EMC virus on A549 cells. The ED50 of each lot is greater than or equal to 2.0 x 108 units/mg. |
Source / Purification
Background
The human IFN-α 2 subvariants 2a and 2b differ by one or two amino acids at positions 23 and/or 34 of the mature protein (4). Pegylated forms of IFN-α 2 and recombinant IFN-α 2 protein are used clinically as standard treatments for anti-viral and anti-tumor therapies (5). IFN-α 2a and 2b proteins have also been shown to have implications in treating hepatitis C (6).
- Stiehm, E.R. et al. (1982) Ann Intern Med 96, 80-93.
- Pellegrini, S. et al. (1989) Mol Cell Biol 9, 4605-12.
- Pfeffer, L.M. and Colamonici, O.R. (1991) Pharmacol Ther 52, 149-57.
- von Gabain, A. et al. (1990) Eur J Biochem 190, 257-61.
- CA Cancer J Clin (1988) 38, 280-90.
- Castelruiz, Y. et al. (1999) Hepatology 29, 1900-4.
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