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Human IL-4 Recombinant Protein #74319

Human IL-4 Recombinant Protein: Image 1
Serial dilutions of Human IL-4 Recombinant Protein were added to TF-1 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.

To Purchase # 74319

Cat. # Size Qty. Price
74319S 20 µg
$275
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Product Information

Storage

Human IL-4 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

Product Description

MW (kDa) 15.1
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-4.
Activity The bioactivity of recombinant hIL-4 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is less than or equal to 0.25 ng/mL.

Source / Purification

Recombinant human IL-4 was expressed in E. coli and is supplied in a lyophilized form.

Background

Interleukin-4 (IL-4) is a cytokine secreted by activated T cells, basophils, and mast cells (1,2). While it contributes to many immunomodulatory responses, it is mainly recognized as the cytokine responsible for eliciting differentiation of naive T cells into Th2 lineage cells that are defined by their secretion of IL-4, IL-5, and IL-10 (3). In addition, IL-4 contributes to immunoglobulin class switching by inducing the production of IgE from B cells (4,5). IL-4 acts through the IL-4 receptor, leading to tyrosine phosphorylation and activation of the Stat6 transcription factor (6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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