Render Target: STATIC
Render Timestamp: 2024-12-23T11:22:55.017Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-04-05 20:28:48.587
Product last modified at: 2024-05-30T07:05:53.571Z
Cell Signaling Technology Logo
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159
  1. Products /
  2. Cytokines & Growth Factors /
  3. Human Interferon-γ (IFN-γ)

Human Interferon-γ (IFN-γ) #3158

We recommend the following alternatives

#

Product Name

80385 Human Interferon-γ (hIFN-γ) Recombinant Protein

Inquiry Info. # 3158

Please see our recommended alternatives.

    Product Information

    Product Usage Information

    Working concentration generally ranges from 10-50 ng/ml.

    Storage

    Lyophilized product is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration ranging from 0.1 to 1 mg/ml which can be further diluted in aqueous solutions as needed. It is recommended to add carrier protein (0.1% HSA or BSA) for long term storage.

    Product Description

    Recombinant human IFN-γ was expressed in E. coli and lyophilized in 10mM Sodium Phosphate buffer pH 7.4. Purity was greater than 95% as determined by RP-HPLC and SDS-PAGE.
    MW (kDa) 16.9
    Purity >95%
    Activity Specific activity as determined by a viral resistance assay was less than 0.05 ng/ml.

    Source / Purification

    Recombinant human IFN-γ expressed in E. coli

    Background

    Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression, and immunoregulation (1). There are three naturally occurring interferons: α, β, and γ. IFN-α is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-α from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-α induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway (2). IFN-α signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (β and ε) (3). All IFN-α signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.

    IFN-γ, also known as type II interferon, is produced mainly in activated T lymphocytes and natural killer cells (4) and has broad effects on various cells of the immune system. Synthesis of IFN-γ is induced by many signaling proteins including IL-2, FGF, and EGF.

    Database Links

    UniProt ID: P01579


    Entrez-Gene Id: 3458


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.