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PDP - Template Name: Growth Factors and Cytokines
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Human Interleukin-21 (hIL-21) #8920

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    Product Information

    Formulation

    With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-21. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

    Storage

    Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
    Maintain sterility. Storage at -20°C should be in a manual defrost freezer.

    Product Description

    MW (kDa) 16
    Purity >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-21. Less than 9% is nicked after Arg114 to generate 6 kDa and 10 kDa polypeptides observed in reduced (+) hIL-21.
    Endotoxin Less than 0.01 ng endotoxin/1 μg hIL-21.
    Activity The bioactivity of recombinant hIL-21 was determined by its ability to induce IFN-γ production by NK-92 cells. The ED50 of each lot is between 1.0-6.0 ng/ml.
    Molecular Formula Recombinant hIL-21 contains no "tags" and the nonglycosylated protein has a calculated MW of 15,463. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptides, with non-reduced having slightly greater mobility due to intramolecular cystines. The expected amino-terminal QGQDR of recombinant hIL-21 was verified by amino acid sequencing.

    Source / Purification

    Recombinant human IL-21 (hIL-21) Gln30-Ser155 (Accession # NM_021803) was expressed in human 293 cells at Cell Signaling Technology.

    Background

    IL-21 is produced by Th17, T follicular helper (Tfh) and NKT cells (1-3). Targets include T cells, B cells, NK cells and dendritic cells (1-3,5). IL-21 induces proliferation and activation of NK cells, thereby up-regulating IFN-γ production and cytotoxic activity (1,3,4). IL-21 increases proliferation and survival of CD40 primed B cells (1,2) and appears to have a significant role in plasma cell differentiation (2). IL-21 binds to a complex consisting of IL-21Rα and the common γ chain, γc. IL-21 binding activates the Jak1/Jak3, Stat1, Stat3 and Stat5 pathway. IL-21 binding can also activate the MAP kinase and PI3K/Akt pathways.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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