Human Interleukin-4 (hIL-4) #8919
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Product Name
Inquiry Info. # 8919
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Product Information
Product Usage Information
With carrier: Add sterile PBS, or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final hIL-4 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.
Carrier free: Add sterile PBS, or PBS containing protein to minimize absorption of hIL-4 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hIL-4 should be greater than 50 μg/ml.
Formulation
Storage
Product Description
| MW (kDa) | 20 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-4. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg hIL-4. |
| Activity | The bioactivity of recombinant hIL-4 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 80-250 pg/ml. |
| Molecular Formula | Recombinant hIL-4 does not have a Met on the amino terminus and the nonglycosylated protein has a calculated MW of 14,963. DTT-reduced and non-reduced protein migrate as larger 20 kDa polypeptides due to glycosylation, with non-reduced having slightly greater mobility due to an intramolecular cystine. The expected amino-terminal HKCDI of recombinant hIL-4 was verified by amino acid sequencing. |
Source / Purification
Background
Limited Uses
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