Human Interleukin-6 Receptor α (hIL-6Rα) #12786
Product Information
Product Usage Information
With carrier: Add sterile 20 mM Tris, pH 7.2 or 20 mM Tris, pH 7.2 containing 1% bovine or human serum albumin or 5-10% FBS to a final hIL-6Rα concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.
Carrier free: Add sterile 20 mM Tris, pH 7.2 or 20 mM Tris, pH 7.2 containing protein to minimize absorption of hIL-6Rα to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock hIL-6Rα should be greater than 50 μg/ml.
Formulation
hIL-6Ra. Carrier free: Lyophilized from a 0.22 μm filtered solution of hIL-6Rα in 20 mM Tris, pH 7.2.
Storage
Product Description
| MW (kDa) | 38 |
| Purity | >90% as determined by SDS-PAGE of 6 μg reduced (+) and nonreduced (-) recombinant hIL-6Rα. All lots are greater than 90% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg hIL-6Rα. |
| Activity | The activity of hIL-6Rα was determined by assessing its ability to enhance IL-6 mediated inhibition of M1 cells proliferation. The ED50 of each lot is between 2 and 15 ng/ml. |
| Molecular Formula | Molecular Characterization: Recombinant hIL-6Rα has a calculated MW of 37,871. DTT-reduced and non-reduced protein migrate as 66 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal LAPRR of recombinant hIL-6Rα was verified by amino acid sequencing. |
Source / Purification
Background
Limited Uses
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