Human/Mouse Indian Hedgehog (Ihh) Recombinant Protein #71711
Product Information
Storage
Human/Mouse Indian Hedgehog (Ihh) Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile 10mM HCl at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 19.9 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg h/mIhh. |
Source / Purification
Background
- Marigo, V. et al. (1995) Genomics 28, 44-51.
- van den Brink, G.R. et al. (2004) Nat Genet 36, 277-82.
- Kumar, S. et al. (1996) Genetics 142, 965-72.
- Nusse, R. and Varmus, H.E. (1992) Cell 69, 1073-87.
- Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810.
- Komiya, Y. and Habas, R. (2008) Organogenesis 4, 68-75.
- Vortkamp, A. et al. (1996) Science 273, 613-22.
Limited Uses
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