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Render Timestamp: 2024-11-22T12:05:58.412Z
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XML generation date: 2024-10-08 19:16:11.376
Product last modified at: 2024-10-09T07:00:58.360Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human PDGF-BB Recombinant Protein #50611

    Product Information

    Storage

    Human PDGF-BB Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 24.9
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hPDGF-BB.
    Activity The bioactivity of recombinant hPDGF-BB was determined in a 3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 2 ng/mL.

    Source / Purification

    Recombinant human PDGF-BB was expressed in E. coli and is supplied in a lyophilized form.

    Background

    PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis (1,2). PDGF-BB is expressed by vascular endothelium, megakaryocytes, and Leydig cells (2). PDGF-BB targets pericytes, fibroblasts, monocytes, and other cell types (1-3). PDGF induces fibroblast growth and migration (3) and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB (1,2). PDGF-BB binds to PDGFRβ and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCγ pathways (1,2). PDGF-BB may have a role in some cancer types (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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