Human TPO Recombinant Protein (mammalian derived) #86116
Product Information
Storage
Human TPO Recombinant Protein (mammalian derived) is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 36 |
| Purity | A greater than or equal to 90% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hTPO. |
| Activity | The bioactivity of recombinant hTPO was determined in an M-O7e cell proliferation assay. The ED50 of each lot is less than or equal to 15 ng/ml. |
Source / Purification
Recombinant human TPO was expressed in genetically modified CHO cells and is supplied in a lyophilized form.
Background
Thrombopoietin (TPO) is a 332-amino acid glycoprotein produced by the liver, kidney, marrow stroma, and several other tissues (1). This cytokine is responsible for the regulation of platelet production and promotes both the proliferation of megakaryocyte progenitors and their maturation into platelet-producing megakaryocytes by binding to the thrombopoietin receptor (TPOR, c-Mpl) (2-5). The concentration of TPO in the bloodstream is regulated by the TPOR on platelets and megakaryocytes (6). Binding of TPO to the TPOR induces receptor dimerization that leads to phosphorylation and activation of the tyrosine kinase Jak2 (7). Studies have suggested that TPO may be a useful cytokine for the prevention and treatment of thrombocytopenia in cancer patients and for developing new strategies to therapeutically intervene in hematopoiesis (1,5).
- Kaushansky, K. (2003) J Thromb Haemost 1, 1587-92.
- Lok, S. et al. (1994) Nature 369, 565-8.
- Kaushansky, K. et al. (1994) Nature 369, 568-71.
- Wendling, F. et al. (1994) Nature 369, 571-4.
- Prow, D. and Vadhan-Raj, S. (1998) Oncology (Williston Park) 12, 1597-604, 1607-8; discussion 1611-4.
- Kato, T. et al. (1998) Stem Cells 16, 322-8.
- Gurney, A.L. and de Sauvage, F.J. (1996) Stem Cells 14 Suppl 1, 116-23.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.