Human TPO Recombinant Protein (mammalian derived) #86116
Product Information
Storage
Human TPO Recombinant Protein (mammalian derived) is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 36 |
| Purity | A greater than or equal to 90% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hTPO. |
| Activity | The bioactivity of recombinant hTPO was determined in an M-O7e cell proliferation assay. The ED50 of each lot is less than or equal to 15 ng/ml. |
Source / Purification
Background
- Kaushansky, K. (2003) J Thromb Haemost 1, 1587-92.
- Lok, S. et al. (1994) Nature 369, 565-8.
- Kaushansky, K. et al. (1994) Nature 369, 568-71.
- Wendling, F. et al. (1994) Nature 369, 571-4.
- Prow, D. and Vadhan-Raj, S. (1998) Oncology (Williston Park) 12, 1597-604, 1607-8; discussion 1611-4.
- Kato, T. et al. (1998) Stem Cells 16, 322-8.
- Gurney, A.L. and de Sauvage, F.J. (1996) Stem Cells 14 Suppl 1, 116-23.
Limited Uses
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