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XML generation date: 2024-09-20 06:23:04.099
Product last modified at: 2024-11-14T13:45:10.381Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159
  1. Products /
  2. Cytokines & Growth Factors /
  3. Human VEGF-165 Recombinant Protein

Human VEGF-165 Recombinant Protein #48143

    Product Information

    Storage

    Human VEGF-165 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 19.3
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hVEGF-165.
    Activity The bioactivity of recombinant hVEGF-165 was determined in a HUVEC cell proliferation assay. The ED50 of each lot is less than or equal to 10 ng/ml.

    Source / Purification

    Recombinant human VEGF-165 was expressed in E. coli and is supplied in a lyophilized form.

    Background

    VEGF-165 is the most abundant splice variant of VEGF-A (1,2). VEGF-165 is produced by a number of cells including endothelial cells, macrophages, and T cells. VEGF-165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration, and vascular permeability (1). VEGF gene expression is induced by hypoxia, inflammatory cytokines, and oncogenes (1,2). VEGF-165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (2,3). VEGF-165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2 (1). VEGF-165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 (1-3) that function to enhance VEGFR2 signaling (1). Binding of VEGF-165 to VEGFR1 and VEGFR2 leads to activation of PI3K/Akt, p38 MAPK, FAK and paxillin (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).

    Database Links

    UniProt ID: P15692-1


    Entrez-Gene Id: 7422


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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