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Product last modified at: 2024-07-22T15:30:07.994Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Mouse GM-CSF Recombinant Protein #70343

    Product Information

    Storage

    Mouse GM-CSF Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 14.3
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg mGM-CSF.
    Activity The bioactivity of recombinant mGM-CSF was determined in an FDC-P1 cell proliferation assay. The ED50 of each lot is less than or equal to 0.05 ng/ml.

    Source / Purification

    Recombinant mouse GM-CSF was expressed in E. coli and is supplied in a lyophilized form.

    Background

    Granulocyte-macrophage colony-stimulating factor (GM-CSF) is produced by activated T cells, NK cells, and macrophages (1,2). Target cells include granulocytes, monocyte precursors, and subsets of differentiated myeloid cells (1,3,4). Many target cells require GM-CSF for survival. GM-CSF induces proliferation, is involved in the hematopoietic differentiation of dendritic cells, and is a key factor in differentiation pathways leading from stem cells. GM-CSF activates effector functions of myeloid cells, thereby linking adaptive and innate immunity and in turn may boost anti-tumor immunity (5). GM-CSF receptor is composed of GM-CSFRα and the common β chain, βC, which is also utilized by IL-3 and IL-5 (1). Binding of GM-CSF initiates the Jak2, Stat5, and PI3K/Akt pathways (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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