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Mouse IL-1β Recombinant Protein #92344

Mouse IL-1β Recombinant Protein: Image 1
The purity of Mouse IL-1β Recombinant Protein was determined by SDS-PAGE of 1 μg non-reduced (-) and reduced (+) recombinant mIL-1β and staining with Coomassie Blue.

To Purchase # 92344

Cat. # Size Qty. Price
92344S 10 µg
$233

Product Information

Storage

Mouse IL-1β Recombinant Protein is supplied as a lyophilized material that is very stable at -20ºC. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

Product Description

MW (kDa) 17.5
Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg mIL-1β.
Activity The bioactivity of recombinant mouse IL-1β (mIL-1β) was determined in a D10S cell proliferation assay. The ED50 of each lot is less than or equal to 0.015 ng/mL.

Source / Purification

Recombinant mIL-1β was expressed in E. coli and is supplied in a lyophilized form.

Background

Interleukin-1β (IL-1β) is a pro-inflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1, and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP), and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII that lacks an intracellular signaling domain, thereby serving as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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