Mouse Transforming Growth Factor β1 (mTGF-β1) #5231
Inquiry Info. # 5231
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Product Information
Product Usage Information
Reconstitution:
With carrier: Add sterile 20 mM citrate, pH 3.0 to a final mTGF-β1 concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.
Carrier free: Add sterile 20 mM citrate, pH 3.0, or 20 mM citrate, pH 3.0 containing protein to minimize absorption of mTGF-β1 to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock mTGF-β1 should be greater than 50 μg/ml.
Formulation
Storage
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Product Description
| MW (kDa) | 13 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mTGF-β1. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg mTGF-β1. |
| Activity | The bioactivity of recombinant mTGF-β1 was determined by assessing inhibition of IL-4 induced HT-2 cell proliferation. The ED50 of each lot is between 40-200 pg/ml. |
| Molecular Formula | Recombinant mTGF-β1 contains no "tags" and the nonglycosylated protein has a calculated MW of 12,810. DTT-reduced protein migrates as a 13 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 25 kDa protein. The expected amino-terminal ALDTN of recombinant mTGF-β1 was verified by amino acid sequencing. |
Source / Purification
Background
Limited Uses
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