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PathScan® Acetyl-α-Tubulin Sandwich ELISA Kit #7204

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  • ELISA
ELISA Image 1: PathScan® Acetyl-α-Tubulin Sandwich ELISA Kit
Figure 1. Treatment of COS cells with Trichostatin A (TSA) increases the acetylation of α-tubulin detected by PathScan® Acetyl-alpha-Tubulin Sandwich ELISA Kit #7204. TSA treatment does not affect the level of α-tubulin that is detected by Western analysis. COS cells (70-80% confluent) were treated for 16-18 hours with 0.4 micromolar TSA at 37oC. Absorbance readings at 450 nm are shown in the top figure while the corresponding Western blots using α-Tubulin Antibody #2125 (left panel) or Acetylated-Lysine Antibody #9441 (right panel) are shown in the bottom figure.

To Purchase # 7204

Cat. #

Size

7204C
1 Kit
7204V1
5 Kits
7204V2
10 Kits
7204V3
20 Kits
7204V4
50 Kits

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H M Mk
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Mk-Monkey 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
Tubulin Mouse mAb Microwells #7746396 tests
Acetylated-Lysine Rabbit Detection mAb #139681 eaGreen (Lyophilized)
Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated) #132721 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

CST's PathScan® Acetyl-α-Tubulin Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of acetylated α-tubulin protein. An α-tubulin mouse mAb has been coated onto the microwells. After incubation with cell lysates, α-tubulin protein (acetylated and non-acetylated) is captured by the coated antibody. Following extensive washing, an acetyl-lysine rabbit Ab is added to detect the acetylated α-tubulin protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of acetylated α-tubulin protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

CST's PathScan® Acetyl-α-Tubulin Sandwich ELISA Kit #7204 detects endogenous levels of acetylated α-tubulin. As shown in Figure 1 using the Acetyl-α-Tubulin Sandwich ELISA Kit #7204, a high level of acetylated α-tubulin is detected in COS cells when treated with TSA. The level of total α-tubulin (acetylated and non-acetylated) remains unchanged as shown by Western analysis (Figure 1). Similar results are obtained when NIH/3T3 and Jurkat cells are treated with TSA (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse, Monkey

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
For Research Use Only. Not For Use In Diagnostic Procedures.
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