PathScan® Acetyl-Histone H4 (Lys12) Sandwich ELISA Kit #7228
- ELISA
To Purchase # 7228
| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 7228C | 1 Kit 96 assays | $641 | |
| 7228V1 | 5 Kits 480 assays | $3,125 | |
| 7228V2 | 10 Kits 960 assays | $6,090 | |
| 7228V3 | 20 Kits 1920 assays | $11,859 | |
| 7228V4 | 50 Kits 4800 assays | $28,845 |
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
Supporting Data
| REACTIVITY | H M |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Product Includes
- Related Products
| Product Includes | Volume | Solution Color |
|---|---|---|
| Histone H4 Mouse mAb Coated Microwells #93097 | 96 tests | |
| Acetyl-Histone H4 (Lys12) Rabbit Detection mAb #71495 | 1 ea | Green (Lyophilized) |
| Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated) #13272 | 1 ea | Red (Lyophilized) |
| Detection Antibody Diluent #13339 | 11 ml | Green |
| HRP Diluent #13515 | 11 ml | Red |
| TMB Substrate #7004 | 11 ml | |
| STOP Solution #7002 | 11 ml | |
| Sealing Tape #54503 | 2 ea | |
| ELISA Wash Buffer (20X) #9801 | 25 ml | |
| ELISA Sample Diluent #11083 | 25 ml | Blue |
| Cell Lysis Buffer (10X) #9803 | 15 ml |
Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.
The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Product Information
Product Description
The PathScan® Acetyl-Histone H4 (Lys12) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of histone H4 when acetylated at lysine 12. A histone H4 mouse antibody has been coated onto the microwells. After incubation with cell lysates, histone H4 protein (acetylated and non-acetylated) is captured by the coated antibody. Following extensive washing, Acetyl-histone H4 (Lys12) rabbit antibody is added to detect acetylated Lys12 on the histone H4 protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of histone H4 acetylated at Lys12.
*Antibodies in kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
- Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
- Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
- Cheung, P. et al. (2000) Cell 103, 263-71.
- Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
- Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
- Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
- Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
- Dai, J. et al. (2005) Genes Dev 19, 472-88.
Pathways
Explore pathways related to this product.
Limited Uses
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