PathScan® Cleaved PARP (Asp214) Sandwich ELISA Kit #7262
- ELISA
Supporting Data
| REACTIVITY | H M |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
CST's PathScan® Cleaved PARP (Asp214) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of cleaved PARP (Asp214) protein. A cleaved PARP (Asp214) Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, cleaved PARP (Asp214) protein is captured by the coated antibody. Following extensive washing, a biotinylated PARP Rabbit mAb is added to detect the captured cleaved PARP protein. HRP-linked Streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of cleaved PARP (Asp214) protein.
*Antibodies in kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
Limited Uses
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