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FastScan^™ Phospho-TBK1/NAK (Ser172) ELISA Kit

FastScan^™ Phospho-TBK1/NAK (Ser172) ELISA Kit #46948

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ELISA

Supporting Data

REACTIVITY

H R

Application Key:

ELISA-ELISA

Species Cross-Reactivity Key:

H-Human
R-Rat

Product Information

Product Description

The FastScan™ Phospho-TBK1/NAK (Ser172) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of TBK1/NAK when phosphorylated at Ser172. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-TBK1/NAK (Ser172) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-TBK1/NAK (Ser172).

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The FastScan™ Phospho-TBK1/NAK (Ser172) ELISA Kit detects endogenous levels of TBK1/NAK when phosphorylated at Ser172, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Rat

Background

TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1 induces IκB degradation and NF-κB activity through IKKβ. TBK1 may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).

Database Links

UniProt ID: Q9UHD2

Entrez-Gene Id: 29110

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.

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FastScan™ Phospho-TBK1/NAK (Ser172) ELISA Kit #46948