FastScan™ Phospho-YB1 (Ser102) ELISA Kit #42976
- ELISA
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
Supporting Data
| REACTIVITY | H M |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Description
The FastScan™ Phospho-YB1 (Ser102) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of YB1 when phosphorylated at Ser102. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-YB1 (Ser102) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-YB1 (Ser102).
*Antibodies in kit are custom formulations specific to kit.
IMPORTANT: This FastScan™ ELISA Kit requires 4 washes at Step 6 of the protocol.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
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- Jurchott, K. et al. (2003) J. Biol. Chem. 278, 27988-96.
- Mertens, P.R. et al. (1997) J. Biol. Chem. 272, 22905-12.
- Uchiumi, T. et al. (1993) Cell Growth Differ. 4, 147-57.
- Lasham, A. et al. (2000) Gene 252, 1-13.
- Lasham, A. et al. (2003) J. Biol. Chem. 278, 35516-23.
- Homer, C. et al. (2005) Oncogene 24, 8314-25.
- Raffetseder, U. et al. (2003) J. Biol. Chem. 278, 18241-8.
- Chen, C.Y. et al. (2000) Genes Dev. 14, 1236-48.
- Sutherland, B.W. et al. (2005) Oncogene 24, 4281-92.
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