Render Target: STATIC
Render Timestamp: 2024-12-26T10:42:31.324Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-12-10 19:01:17.761
Product last modified at: 2024-09-20T07:05:33.551Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: FastScan ELISA Kit
PDP - Template ID: *******a26362b

FastScan Phospho-Zap-70 (Tyr319) ELISA Kit #47223

Filter:
  • ELISA

Inquiry Info. # 47223

Please see our recommended alternatives.

    Supporting Data

    REACTIVITY H M
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Description

    The FastScan™ Phospho-Zap-70 (Tyr319) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Zap-70 when phosphorylated at Tyr319. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with phospho-Zap-70 (Tyr319) in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of phospho-Zap-70 (Tyr319).
    *Antibodies in kit are custom formulations specific to kit.
    IMPORTANT: This FastScan™ ELISA kit requires 4 washes at Step 6 of the protocol.

    Protocol

    Specificity / Sensitivity

    The FastScan™ Phospho-Zap-70 (Tyr319) ELISA Kit detects endogenous levels of Zap-70 when phosphorylated at Tyr319, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

    Species Reactivity:

    Human, Mouse

    Background

    The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.
    U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.