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PDP - Template Name: FastScan ELISA Kit
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FastScan Total Glucocorticoid Receptor ELISA Kit #88161

Filter:
  • ELISA
ELISA Image 1: FastScan™ Total Glucocorticoid Receptor ELISA Kit
Figure 1. Treatment of HeLa cells with UV stimulates phosphorylation of glucocorticoid receptor at Ser134 and Ser226, but does not affect the level of total glucocorticoid receptor protein. The relationship between lysate protein concentration from untreated and UV-treated HeLa cells and the absorbance at 450 nm using the FastScan™ Total Glucocorticoid Receptor ELISA Kit #88161 is shown in the upper figure. The corresponding western blots using glucocorticoid receptor antibody (left panel), phospho-glucocorticoid receptor (Ser134) antibody (middle panel), and phospho-glucocorticoid receptor (Ser226) antibody (right panel) are shown in the lower figure. HeLa cells were either left untreated or treated with UV (100 J/m2 with 1 hr recovery), and then lysed.

To Purchase # 88161

Cat. # Size Qty. Price Ships
88161C 1 Kit
96 assays
$654
88161V1 5 Kits
480 assays
$3,188
88161V2 10 Kits
960 assays
$6,213
88161V3 20 Kits
1920 assays
$12,099
88161V4 50 Kits
4800 assays
$29,430

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolume (with Count)Solution Color
FastScan™ ELISA Microwell Strip Plate, 96 Well #532571 x 96 tests
Glucocorticoid Receptor Rabbit Capture mAb #327021 x 1 eaGreen (Lyophilized)
Glucocorticoid Receptor Rabbit HRP-linked mAb #424631 x 1 eaRed (Lyophilized)
FastScan™ ELISA Capture Antibody Diluent #160761 x 3 mlGreen
FastScan™ ELISA HRP Antibody Diluent #281201 x 3 ml
TMB Substrate #70041 x 11 ml
STOP Solution #70021 x 11 ml
Sealing Tape #545031 x 1 ea
ELISA Wash Buffer (20X) #98011 x 25 ml
FastScan™ ELISA Cell Extraction Buffer (5X) #699051 x 10 ml
FastScan™ ELISA Cell Extraction Enhancer Solution (50X) #252431 x 1 ml
FastScan™ ELISA Kit #88161 Positive Control Type 1 #206631 x 1 ea

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The FastScan™ Total Glucocorticoid Receptor ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of glucocorticoid receptor. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with glucocorticoid receptor in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of glucocorticoid receptor.

*Antibodies in this kit are custom formulations specific to kit.

IMPORTANT: This FastScan™ ELISA Kit requires 4 washes at Step 6 of the protocol.

Protocol

Specificity / Sensitivity

The FastScan™ Total Glucocorticoid Receptor ELISA Kit detects endogenous levels of glucocorticoid receptor, as shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

Glucocorticoid hormones control cellular proliferation, inflammation, and metabolism through their association with the glucocorticoid receptor (GR)/NR3C1, a member of the nuclear hormone receptor superfamily of transcription factors (1). GR is composed of several conserved structural elements, including a carboxy-terminal ligand-binding domain (which also contains residues critical for receptor dimerization and hormone-dependent gene transactivation), a neighboring hinge region containing nuclear localization signals, a central zinc-finger-containing DNA-binding domain, and an amino-terminal variable region that participates in ligand-independent gene transcription. In the absence of hormone, a significant population of GR is localized to the cytoplasm in an inactive form via its association with regulatory chaperone proteins, such as HSP90, HSP70, and FKBP52. On hormone binding, GR is released from the chaperone complex and translocates to the nucleus as a dimer to associate with specific DNA sequences termed glucocorticoid response elements (GREs), thereby enhancing or repressing transcription of specific target genes (2). It was demonstrated that GR-mediated transcriptional activation is modulated by phosphorylation (3-5). Although GR can be basally phosphorylated in the absence of hormone, it becomes hyperphosphorylated upon binding receptor agonists. It has been suggested that hormone-dependent phosphorylation of GR may determine target promoter specificity, cofactor interaction, strength and duration of receptor signaling, receptor stability, and receptor subcellular localization (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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FastScan™ ELISA is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patents 9,086,407, 9,261,500, and 9,476,874, foreign equivalents, and child patents deriving therefrom.
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