FastScan™ Total LC3B ELISA Kit #35172
- ELISA
To Purchase # 35172
| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 35172C | 1 Kit 96 assays | $654 | |
| 35172V1 | 5 Kits 480 assays | $3,188 | |
| 35172V2 | 10 Kits 960 assays | $6,213 | |
| 35172V3 | 20 Kits 1920 assays | $12,099 | |
| 35172V4 | 50 Kits 4800 assays | $29,430 |
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
Supporting Data
| REACTIVITY | H M R Mk |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Product Includes
- Related Products
| Product Includes | Volume (with Count) | Solution Color |
|---|---|---|
| FastScan™ ELISA Microwell Strip Plate, 96 Well #53257 | 1 x 96 tests | |
| LC3B Rabbit Capture mAb #48369 | 1 x 1 ea | Green (Lyophilized) |
| LC3B Mouse HRP-linked mAb #59959 | 1 x 1 ea | Red (Lyophilized) |
| FastScan™ ELISA Capture Antibody Diluent #16076 | 1 x 3 ml | Green |
| FastScan™ ELISA HRP Antibody Diluent #28120 | 1 x 3 ml | |
| TMB Substrate #7004 | 1 x 11 ml | |
| STOP Solution #7002 | 1 x 11 ml | |
| ELISA Wash Buffer (20X) #9801 | 1 x 25 ml | |
| FastScan™ ELISA Cell Extraction Buffer (5X) #69905 | 1 x 10 ml | |
| FastScan™ ELISA Cell Extraction Enhancer Solution (50X) #25243 | 1 x 1 ml | |
| FastScan™ ELISA Kit #35172 Positive Control Type 2 #47385 | 1 x 1 ea | |
| Sealing Tape #54503 | 1 x 1 ea |
Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.
The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Product Information
Product Description
The FastScan™ Total LC3B ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of LC3B. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with LC3B in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of LC3B.
*Antibodies in kit are custom formulations specific to kit.
IMPORTANT: This FastScan™ ELISA Kit requires 4 washes at Step 6 of the protocol.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.
- Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.
- Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.
- Mann, S.S. and Hammarback, J.A. (1994) J. Biol. Chem. 269, 11492-97.
- Lang, T. et al. (1998) EMBO J. 17, 3597-607.
- Kabeya, Y. et al. (2000) EMBO J. 19, 5720-28.
- He, H. et al. (2003) J. Biol. Chem. 278, 29278-87.
- Tanida, I. et al. (2004) J. Biol. Chem. 279, 47704-10.
- Wu, J. et al. (2006) Biochem. Biophys. Res. Commun. 339, 437-42.
- Ichimura, Y. et al. (2000) Nature 408, 488-92.
- Kabeya, Y. et al. (2004) J. Cell Sci. 117, 2805-12.
Pathways
Explore pathways related to this product.
Limited Uses
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