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PathScan® Neurofilament-L Sandwich ELISA Kit #99175

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  • ELISA
Figure 1. Detection of Neurofilament-L protein in mouse brain, HeLa cells, and rat brain is examined by using the PathScan® Neurofilament Sandwich ELISA Kit. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using Neurofilament-L (C28E10) Rabbit mAb #2837 is shown in the bottom figure.

To Purchase # 99175

Cat. # Size Qty. Price
99175C 1 Kit
96 assays
$641
99175V1 5 Kits
480 assays
$3,125
99175V2 10 Kits
960 assays
$6,090
99175V3 20 Kits
1920 assays
$11,859
99175V4 50 Kits
4800 assays
$28,845

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Product Specifications

REACTIVITY M R
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • M-Mouse 
  • R-Rat 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
Neurofilament-L Mouse mAb Coated Microwells #4600796 tests
Neurofilament-L Rabbit Detection mAb #889021 eaGreen (Lyophilized)
Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated) #132721 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

For the “C” and “V” kits, the supplied 96-well strip plate consists of twelve 8-well strips in a support frame. This enables custom plate configurations.

Product Information

Product Description

The PathScan® Neurofilament-L Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of neurofilament-L protein. A neurofilament-L mouse mAb has been coated onto the microwells. After incubation with cell lysates, the neurofilament-L proteins are captured by the coated antibody. Following extensive washing, an neurofilament-L rabbit detection mAb is added to detect captured neurofilament-L proteins. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of neurofilament-L protein.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan® Neurofilament-L Sandwich ELISA Kit detects endogenous levels of nerofilament-L protein in mouse or rat brains, as shown in Figure 1. This kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Mouse, Rat

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

Alternate Names

AD; Alzheimer; Alzheimer's; elisa; NDG; Neurofilament-L; NF-L; NFL; screen

For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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