PathScan® Phospho-c-Kit (Tyr703) Sandwich ELISA Kit #67303
- ELISA
Supporting Data
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
The PathScan® Phospho-c-Kit (Tyr703) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of c-Kit protein phosphorylated at Tyr703. A c-Kit mouse antibody has been coated onto the microwells. After incubation with cell lysates, c-Kit protein is captured by the coated antibody. Following extensive washing, a phospho-c-Kit (Tyr703) rabbit detection antibody is added to detect the captured phospho-c-Kit (Tyr703) protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of c-Kit phosphorylated at Tyr703.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
Tyr703 is also located in the kinase insert of c-Kit. Its phosphorylation provides a docking site for Grb2 binding (8). It was demonstrated that Tyr703 was constitutively phosphorylated in GIST tumor cells (9).
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- Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
- Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
- Nocka, K. et al. (1990) EMBO J 9, 1805-13.
- Hirota, S. et al. (1998) Science 279, 577-80.
- Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
- Thömmes, K. et al. (1999) Biochem J 341 (Pt 1), 211-6.
- Duensing, A. et al. (2004) Oncogene 23, 3999-4006.
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