Render Target: STATIC
Render Timestamp: 2025-01-22T05:00:42.547Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-12-25 01:36:42.906
Product last modified at: 2025-01-01T09:02:47.533Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: ELISA Kit
PDP - Template ID: *******bd382c2

PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit #7176

Filter:
  • ELISA
ELISA Image 1: PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit
Figure 1: Treatment of HeLa cells with Interleukin-4 stimulates phosphorylation of cdc2 at Tyr15 as detected by PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA kit #7176, but does not affect the level of total cdc2 protein detected by cdc2 Antibody (#9116). OD 450 readings are shown in the top figure, while the corresponding Western blots using Phospho-cdc2 (Tyr15) Antibody #9111 (left panel) or cdc2 Antibody #9116 (right panel) is shown in the bottom figure.

To Purchase # 7176

Cat. # Size Qty. Price Ships
7176C 1 Kit
96 assays
$641
7176V1 5 Kits
480 assays
$3,125
7176V2 10 Kits
960 assays
$6,090
7176V3 20 Kits
1920 assays
$11,859
7176V4 50 Kits
4800 assays
$28,845

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
Phospho-Cdc2 (Tyr15) Rabbit mAb Coated Microwells #6303096 tests
cdc2 Mouse Detection mAb #931911 eaGreen (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) #133041 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

CST's PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-cdc2 (Tyr15) protein. A Phospho-cdc2 (Tyr15) Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, phospho-cdc2 (Tyr15) protein is captured by the coated antibody. Following extensive washing, cdc2 Mouse mAb is added to detect the captured phospho-cdc2 protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of phospho-cdc2 (Tyr15) protein.

*Antibodies in kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

CST's PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit detects endogenous levels of phospho-cdc2 (Tyr15) protein. As shown in Figure 1, using the Phospho-cdc2 (Tyr15) ELISA Kit #7176, a significant induction of phospho-cdc2 (Tyr15) is detected in HeLa cells treated with Interleukin-4. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.