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PDP - Template Name: ELISA Kit
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  1. Products /
  2. ELISA Kits /
  3. PathScan® Phospho-HER2/ErbB2 (panTyr) Sandwich ELISA Kit

PathScan® Phospho-HER2/ErbB2 (panTyr) Sandwich ELISA Kit #7968

Filter:
  • ELISA
ELISA Image 1: PathScan® Phospho-HER2/ErbB2 (panTyr) Sandwich ELISA Kit
Figure 1: Constitutive phosphorylation of HER2/ErbB2 in Calu-3 cells lysed in the presence of phosphatase inhibitors* (phospho lysate) is detected by PathScan® Phospho-HER2/ErbB2 (panTyr) Sandwich ELISA Kit #7968 (top, right). In contrast, a low level of phospho-HER2/ErbB2 protein is detected in Calu-3 cells lysed in the absence of phosphatase inhibitors* (nonphospho lysate). Similar levels of total HER2/ErbB2 protein from both nonphospho or phospho lysates are detected by PathScan® Total HER2/ErbB2 Sandwich ELISA Kit #7310 (top, left). Absorbance at 450 nm is shown in the top figure while corresponding western blots using Phospho-HER2/ErbB2 (Tyr1221/1222) Rabbit mAb #2243 (right) or a total HER2/ErbB2 Antibody #2242 (left) are shown in the bottom figure. *Phosphatase inhibitors include sodium pyrophosphate, β-glycerophosphate and Na3VO4.
1/2

To Purchase # 7968

Cat. # Size Qty. Price
7968C 1 Kit
96 assays
$641
7968V1 5 Kits
480 assays
$3,125
7968V2 10 Kits
960 assays
$6,090
7968V3 20 Kits
1920 assays
$11,859
7968V4 50 Kits
4800 assays
$28,845

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
HER2/ErbB2 Rabbit mAb Coated Microwells #4701796 tests
Phospho Tyrosine Mouse Detection mAb #129821 eaGreen (Lyophilized)
Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated) #133041 eaRed (Lyophilized)
Detection Antibody Diluent #1333911 mlGreen
HRP Diluent #1351511 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
ELISA Sample Diluent #1108325 mlBlue
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The PathScan® Phospho-HER2/ErbB2 (panTyr) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of tyrosine-phosphorylated HER2/ErbB2 protein. A HER2/ErbB2 rabbit mAb has been coated on the microwells. After incubation with cell lysates, HER2/ErbB2 protein (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a phospho-tyrosine mouse detection antibody is added to detect captured tyrosine-phosphorylated HER2/ErbB2 protein. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of HER2/ErbB2 protein phosphorylated on tyrosine.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

PathScan® Phospho-HER2/ErbB2 (panTyr) Sandwich ELISA Kit #7968 detects endogenous levels of HER2/ErbB2 protein only when phosphorylated at Tyr residues (see Figure 1). The kit sensitivity is shown in Figure 2. This kit does not cross-react with other proteins of ErbB family (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human

Background

The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).

Pathways

Explore pathways related to this product.


Database Links

UniProt ID: P04626


Entrez-Gene Id: 2064


Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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