PathScan® Phospho-HER3/ErbB3 (Tyr1328) Sandwich ELISA Kit #35585
- ELISA
Supporting Data
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
Investigators have found that ErbB3 is highly expressed in many cancer cells (5) and activation of the ErbB3/PI3K pathway is correlated with malignant phenotypes of adenocarcinomas (6). Research studies have demonstrated that in tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members (e.g., ErbB2 requires ErbB3 to drive breast tumor cell proliferation) (7). Thus, investigators view inhibiting interaction between ErbB3 and ErbB tyrosine kinases as a novel strategy for anti-tumor therapy.
Phosphorylation of Tyr1328 on HER3/ErbB3 was identified at Cell Signaling Technology using PTMScan®, an LC-MS/MS platform for modified site discovery from Cell Signaling Technology (8).
- Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2, 127-37.
- Guy, P.M. et al. (1994) Proc Natl Acad Sci USA 91, 8132-6.
- Songyang, Z. et al. (1993) Cell 72, 767-78.
- Kim, H.H. et al. (1994) J Biol Chem 269, 24747-55.
- Sithanandam, G. et al. (2003) Carcinogenesis 24, 1581-92.
- Kobayashi, M. et al. (2003) Oncogene 22, 1294-301.
- Holbro, T. et al. (2003) Proc Natl Acad Sci USA 100, 8933-8.
- Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
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