PathScan® Phospho-HSP27 (Ser78) Sandwich ELISA Kit #7290
Important Ordering Details
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Supporting Data
| REACTIVITY | H Mk |
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Description
*Antibodies in kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
CST's PathScan® Phospho-HSP27 (Ser78) Sandwich ELISA Kit detects endogenous levels of phospho-HSP27 (Ser78) protein. Using this Sandwich ELISA Kit #7290, a significant induction of phospho-HSP27 (Ser78) can be detected in HeLa cells treated with UV light. However, the level of total HSP27 (phospho and non-phospho), detected by the Total HSP27 Sandwich ELISA Kit #7295, remains unchanged (Figure 1). COS cells treated with UV light show similar results (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human, Monkey
Background
Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).
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