PathScan® Phospho-PTEN (Ser380) Sandwich ELISA Kit #7285
- ELISA
Supporting Data
| REACTIVITY | H M Mk |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Description
CST's PathScan® Phospho-PTEN (Ser380) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-PTEN (Ser380) protein. A PTEN Mouse mAb has been coated onto the microwells. After incubation with cell lysates, PTEN protein (phospho and non-phospho) is captured by the coated antibody. Following extensive washing, Phospho-PTEN (Ser380/Thr382/383) Antibody is added to detect the captured phospho-PTEN (Ser380). Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for this developed color is proportional to the quantity of phospho-PTEN (Ser380) protein.
*Antibodies in kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
- Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7.
- Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8.
- Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11.
- Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91.
- Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8.
- Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8.
- Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30.
- Funamoto, S. et al. (2002) Cell 109, 611-23.
- Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
Product Citations: 1
Limited Uses
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