PathScan® Phospho-SMAD2 (Ser465/467) Sandwich ELISA Kit #7348
- ELISA
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
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Supporting Data
| REACTIVITY | H M Mi |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mi-Mink
Product Information
Product Description
The PathScan® Phospho-SMAD2 (Ser465/467) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of SMAD2 when phosphorylated at Ser465/467. A SMAD2 Mouse monoclonal Antibody has been coated onto the microwells. After incubation with cell lysates, SMAD2 (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Phospho-SMAD2 (Ser465/467) Detection Antibody is added to detect serine phosphorylation of the captured SMAD2 protein. Anti-rabbit IgG, HRP-Linked Ab is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of SMAD2 phosphorylated at Ser465/467.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Heldin, C.H. et al. (1997) Nature 390, 465-71.
- Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
- Derynck, R. et al. (1998) Cell 95, 737-40.
- Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
- Whitman, M. (1998) Genes Dev 12, 2445-62.
- Wrana, J.L. (2000) Sci STKE 2000, re1.
- Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
- Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.
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