PathScan® RP BRD4 Sandwich ELISA Kit #77195
- ELISA
Supporting Data
| REACTIVITY | H M R Mk |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Description
The rapid protocol (RP) PathScan® RP BRD4 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of BRD4 protein in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with BRD4 protein in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of BRD4 protein. Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Belkina, A.C. and Denis, G.V. (2012) Nat Rev Cancer 12, 465-77.
- Voigt, P. and Reinberg, D. (2011) Genome Biol 12, 133.
- Wu, S.Y. and Chiang, C.M. (2007) J Biol Chem 282, 13141-5.
- Dey, A. et al. (2003) Proc Natl Acad Sci U S A 100, 8758-63.
- Dey, A. et al. (2009) Mol Biol Cell 20, 4899-909.
- Zhao, R. et al. (2011) Nat Cell Biol 13, 1295-304.
- Jang, M.K. et al. (2005) Mol Cell 19, 523-34.
- Yang, Z. et al. (2005) Mol Cell 19, 535-45.
- Dawson, M.A. et al. (2011) Nature 478, 529-33.
- Muller, S. et al. (2011) Expert Rev Mol Med 13, e29.
- Mertz, J.A. et al. (2011) Proc Natl Acad Sci U S A 108, 16669-74.
- Filippakopoulos, P. et al. (2010) Nature 468, 1067-73.
- Nicodeme, E. et al. (2010) Nature 468, 1119-23.
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