PathScan® RP Cleaved Gasdermin D (Asp276) Sandwich ELISA Kit #25566
- ELISA
Important Ordering Details
Custom Ordering Details:If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.
Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.
Supporting Data
| REACTIVITY | M |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
The rapid protocol (RP) PathScan® RP Cleaved Gasdermin D (Asp276) Sandwich ELISA kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of the amino fragment of Gasdermin D protein when cleaved at Asp276 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with cleaved Gasdermin D (Asp276) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of cleaved Gasdermin D (Asp276). Learn more about your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
- Kayagaki, N. et al. (2015) Nature 526, 666-71.
- Shi, J. et al. (2015) Nature 526, 660-5.
- Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20.
- Aglietti, R.A. et al. (2016) Proc Natl Acad Sci U S A 113, 7858-63.
- Ding, J. et al. (2016) Nature 535, 111-6.
- Liu, X. et al. (2016) Nature 535, 153-8.
- Sborgi, L. et al. (2016) EMBO J 35, 1766-78.
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