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PathScan® RP Phospho-c-Fos (Ser32) Sandwich ELISA Kit #78232

Filter:
  • ELISA
ELISA Image 1: PathScan® RP Phospho-c-Fos (Ser32) Sandwich ELISA Kit
Figure 1. Treatment of HeLa cells with 12-O-Tetradecanoylphorbol-13-Acetate (TPA) stimulates phosphorylation of c-Fos protein at Ser32. The relationship between lysate protein concentration from untreated and TPA-treated HeLa cells and the absorbance at 450 nm using the PathScan® RP Phospho-c-Fos (Ser32) Sandwich ELISA Kit #78232 is shown in the upper figure. The corresponding western blots using phospho-c-Fos (Ser32) antibody (left panel) and α-Actinin antibody (right panel) are shown in the lower figure. After serum starvation, HeLa cells were either left untreated or treated with TPA (200 nM) for 4 hr at 37°C, and then lysed.

To Purchase # 78232

Cat. # Size Qty. Price Ships
78232C 1 Kit
96 assays
$641 Jan 22
78232V1 5 Kits
480 assays
$3,125 Jan 22
78232V2 10 Kits
960 assays
$6,090 Jan 22
78232V3 20 Kits
1920 assays
$11,859 Jan 22
78232V4 50 Kits
4800 assays
$28,845 Feb 12

Important Ordering Details

Custom Ordering Details:

If kit quantities from the same lot are needed in unlisted sizes, contact us for processing time and pricing.

Looking for this ELISA kit in a 384-well format? Inquire for availability, processing time, and pricing.

Supporting Data

REACTIVITY H M
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Product Includes
  • Related Products
Product IncludesVolumeSolution Color
c-Fos Rabbit mAb Coated Microwells #9106496 tests
Phospho-c-Fos (Ser32) Rabbit Detection mAb #849541 eaRed (Lyophilized)
HRP Diluent #135155.5 mlRed
TMB Substrate #700411 ml
STOP Solution #700211 ml
Sealing Tape #545032 ea
ELISA Wash Buffer (20X) #980125 ml
Cell Lysis Buffer (10X) #980315 ml

Kit contents scale proportionally with size, except sealing tape.
Example: The V1 kit contains 5X the listed quantities above, but will exclude the sealing tape.

The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.

Product Information

Product Description

The rapid protocol (RP) PathScan® RP Phospho-c-Fos (Ser32) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of c-Fos protein phosphorylated at Ser32 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-c-Fos (Ser32) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of phospho-c-Fos (Ser32). Learn more about your ELISA kit options here.

*Antibodies in this kit are custom formulations specific to kit.

Protocol

Specificity / Sensitivity

The PathScan® RP Phospho-c-Fos (Ser32) Sandwich ELISA Kit detects endogenous levels of c-Fos protein phosphorylated at Ser32. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Species Reactivity:

Human, Mouse

Background

The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).

Pathways

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