PathScan® RP Phospho-HS1 (Tyr378/397) Sandwich ELISA Kit #74506
- ELISA
Supporting Data
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
The rapid protocol (RP) PathScan® RP Phospho-HS1 (Tyr378/397) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of HS1 protein phosphorylated at Tyr378/397 in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-HS1 (Tyr378/397) in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of phospho-HS1 (Tyr378/397). Learn more about your ELISA kit here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
Species Reactivity:
Background
HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).
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- Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
- Gomez, T.S. et al. (2006) Immunity 24, 741-52.
- Kahner, B.N. et al. (2007) Blood 110, 2449-56.
- Yamanashi, Y. et al. (1997) J Exp Med 185, 1387-92.
- Hao, J.J. et al. (2004) J Biol Chem 279, 33413-20.
- Brunati, A.M. et al. (2005) J Biol Chem 280, 21029-35.
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