PathScan® RP Phospho-LAT (Tyr220) Sandwich ELISA Kit #7936
- ELISA
Supporting Data
| REACTIVITY | H |
Application Key:
- ELISA-ELISA
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
The rapid protocol (RP) PathScan® RP Phospho-LAT (Tyr220) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-LAT (Tyr220) in a reduced assay time of 1.5 hours. Incubation of cell lysate and detection antibody on the capture antibody coated microwell plate forms a sandwich with LAT protein phosphorylated at Tyr220 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of LAT protein phosphorylated at Tyr220. Learn more about all of your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
The PathScan® RP Phospho-LAT (Tyr220) Sandwich ELISA Kit #7936 detects endogenous levels of phospho-LAT when phosphorylated at Tyr191. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Background
Limited Uses
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